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Studies of neuronal, endocrine, and metabolic disorders would be facilitated by characterization of the hypothalamus proteome. Protein extracts prepared from 16 whole rat hypothalami were measured by data-independent label-free nano LC-MS/MS. Peptide features were detected, aligned, and searched against a rat Swiss-Prot database using ProteinLynx Global Server v.2.5. The final combined dataset comprised 21 455 peptides, corresponding to 622 unique proteins, each identified by a minimum of two distinct peptides. The majority of the proteins (69%) were cytosolic, and 16% were membrane proteins. Important proteins involved in neurological and synaptic function were identified including several members of the Ras-related protein family and proteins involved in glutamate biosynthesis.

Original publication

DOI

10.1002/pmic.201100642

Type

Journal

Proteomics

Publication Date

11/2012

Volume

12

Pages

3386 - 3392

Keywords

Animals, Biomarkers, Chromatography, Liquid, Databases, Protein, Hypothalamus, Isoelectric Point, Nerve Tissue Proteins, Peptide Fragments, Proteome, Proteomics, Rats, Rats, Wistar, Tandem Mass Spectrometry